Centre-back Giorgio Cheillini has been ruled out of Italy's quarter-final against England in Kiev.
The 27-year-old Juventus defender had limped out of Monday's final Group C game with a hamstring problem.
Chiellini underwent tests on Wednesday morning but Italy team doctor Enrico Castellacci then said he wouldn't be available for Sunday's last eight clash.
"We'll do everything to get him back as soon as possible, we're not setting limits. If we make progress we'll try to get him back for the first meaningful match," said Castellacci.
"Chiellini is very determined to recover, we have to try, we've no other options."
The rugged centre-half will likely be replaced by club-mate Leonardo Bonucci against Roy Hodgson's team.
Italian defender Giorgio Chiellini asks for medical assistance during the Euro 2012 football championships match Italy vs Republic of Ireland on June 18, 2012 at the Municipal Stadium in Poznan. AFP PHOTO / GIUSEPPE CACACE